Z Averages as mentioned earlier is the output of a method called Cumulants, which. The technique operates by cross-correlating scattered light of two different colours. For aggregated protein samples, DLS results can show that the aggregate distribution in the sample is polydisperse, but it is not feasible to obtain detailed quantitative information on monomer and aggregate content. DLS correlation data can be converted to size information in a number of ways. This presentation will be useful for SZ-100 Nanoparticle Analyzer users and anyone who would like to become more comfortable with DLS data. Particle concentrations cannot be obtained by DLS. Learn how dynamic light scattering instruments collect data and how that data is transformed into particle size information including central values and distributions. For protein monomers and aggregates, a size range from about 1 nm to several µm can be covered. In dynamic light scattering (DLS), the time-dependent fluctuations in the scattered light are measured by a single photon counting module. The particle size range of DLS depends on the properties of the analyzed species, such as refractive index or density, as well as the surrounding formulation, mainly the viscosity. The technique can also be employed for the analysis of colloidal systems, such as liposomes, nanoparticles, polymers and virus-like particles. DLS can determine the hydrodynamic size of protein monomers, small aggregates in the nanometer range and partially also particles in the high nanometer/low micrometer range. measurement and interpretation of light scattering data on a microsecond time scale. Brown, W., Oxford Science Publication, Oxford, 1993, Chpt.4, pp. Based on intensity fluctuations of laser light scattered by the molecules/particles, moving in Brownian motion, the diffusion coefficient is determined and converted to particle size via the Stokes-Einstein equation. tpnek, P., Data Analysis in Dynamic Light Scattering, in Dynamic Light Scattering, Ed.
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